Abstract: To study a convenient, safe and non-ionizing method for fracture detection, a total focusing method for ultrasonic double-layer refraction correction is proposed. Starting from the signal processing and imaging algorithm, the cross-correlation calculation between echo signal and standard emission signal is performed to obtain echo signal with very low noise. Then, the feasibility of total focusing method in homogeneous medium is verified by using the Field Ⅱ crack simulation experiment, and the simulation result shows that the average relative error of crack width is 5.60%. Finally, the full matrix data collection is carried out for the in-vitro bovine tibial plate, and the traditional total focusing method and the double-layer refraction modified total focusing method are used to image the bone fracture. The former method is able to image the fracture with the average relative error 8.33%, however, fails to image the bottom surface of the bone. The latter method not only shows the fracture (relative error is 6.98%) clearly, but also shows the bottom surface of the bone, by which the thickness of the bone is accurately measured (relative error is 5.75%). Therefore, the ultrasonic total focusing method has application potential in the detection of bone fracture.